It is obvious that the difficulties in its www.selleckchem.com/products/Perifosine.html synthesis, the absence of regulatory standards, and toxin counterfeiting have resulted in the marketing of products. Compared with the other reported methods for estimation of DON, this method is simple and quick. A previous study showed that for isolation and increases maximum absorbance in UV range DON was passes through immunoaffinity column, whereas in the present study DON was separated using alumina-silica-activated charcoal (1:1:1), which is more economic and easier. Previously, DON was isolated by Sephadex? LH20 column and other methods, viz, solid phase extraction, liquid extraction, ion-exchange columns, immunoaffinity columns, etc. The new procedure described here for isolation of DON has several advantages: it is economical, simple, and less time consuming than the previous methods for large-scale separation of DON.
HPLC methods are sensitive and more accurate compared with LC�CMS and GC�CMS and most manufacturers prefer the HPLC methods. Besides, LC�CMS and GC�CMS are expensive techniques, and not all manufacturers are able to bear the costs involved. The present investigation presents an alternative to LC�CMS for analysis for DON. The method has a novel approach, using an immunoaffinity column for isolation of DON. CONCLUSIONS DON is a potential toxin found in food grains. HPLC methods can be useful techniques for quality control and monitoring of this contamination. In this article we have described an accurate, sensitive, and easy technique to estimate DON in a food sample.
The present method validation and repeatability compiles with standard. Therefore, the present investigation has importance for alternates of ELISA, LC-MS analysis for DON. ACKNOWLEDGMENTS This research was fully supported by the Defense Research Laboratory, DRDO, who provided all facilities and financial assistance. Footnotes Source of Support: Defense Research Laboratory, DRDO Conflict of Interest: None declared.
Chemically Olmesartan medoxomil is (5-methyl-2-oxo-1,3-dioxol-4-yl)methyl5-(2-hydroxypropan-2-yl)-2-propyl-3-[[4-[2-(2H-tetrazol-5-yl)phenyl]phenyl]methyl]imidazole-4-carboxylate [Figure 1], and is a synthetic analog of the angiotensin II receptor blocker, which is widely utilized nowadays in the first line treatment of hypertension.
[1,2] Figure 1 Structure of Olmesartan medoxomil Analysis is an important component in the formulation development of any drug molecule. A suitable and validated method has to be available for the analysis of drug(s) in bulk, in drug delivery systems, in dissolution studies (in vitro), and in biological samples (in vivo). If such a suitable method for a specific Anacetrapib need is not available, then it becomes essential to develop a simple, sensitive, accurate, precise, and reproducible method for the estimation of drug samples.