Patients were retrospectively divided into 2 groups: those succes

Patients were retrospectively divided into 2 groups: those successfully bridged to transplant (Group A) and those who died (Group B). Patients were also divided into 2 periods of implantation: Group X (2000-2005) and Group Y (2006-2008, which used a multidiscipline selection process).

RESULTS: Overall success rate to transplantation was 71.3%, with Group Y demonstrating an 82% success to transplant rate vs 63% in Group X. One-year actuarial survival after transplant was 89% compared with 92% in patients without a ventricular assist

device. There were no statistically significant laboratory parameters between Groups A and B identifying potential risk factors for poor outcome.

CONCLUSION: Biventricular assist device therapy represents an effective and reliable means of supporting selected Interagency Registry for Mechanically Assisted Circulatory Support profile 1 patients as a bridge to transplantation, with excellent

Semaxanib success to transplant rates and post-transplant survival. J Heart Lung Transplant 2011;30:1143-7 (C) 2011 International Society for Heart and Lung Transplantation. All rights reserved.”
“PURPOSE: To evaluate the outcomes of phacoemulsification with intraocular lens (IOL) implantation in eyes with uveitis.

SETTING: Tertiary care center, Chandigarh, India.

METHODS: Veliparib research buy Consecutive patients with uveitis and visually significant cataract were retrospectively studied

for outcomes after phacoemulsification and implantation of a poly(methyl methacrylate) (PMMA) or a hydrophobic acrylic IOL.

RESULTS: The study comprised 108 eyes of 81 patients (50 women, 31 men) with a mean age of 42.3 years +/- 13.98 (SD) (range 18 to 75 years) and a mean follow-up of 21.95 months (range 12 to 66 months). Etiology of uveitis was presumed tuberculosis (n = 24), Vogt Koyanagi Harada syndrome (n = 9), Behcet disease learn more (n = 8), sarcoidosis (n = 5), ankylosing spondylitis (n = 4), serpiginous choroiditis (n = 2), and idiopathic (n = 29). The mean corrected distance visual acuity (CDVA) was 1.08 +/- 0.85 logMAR preoperatively and 0.42 +/- 0.78 D logMAR postoperatively; the improvement was statistically significant (P<.001, paired t test); Seventy-seven eyes (71.30%) achieved a CDVA between 0.00 log MAR and 0.30 logMAR (20/20 to 20/40 Snellen). Posterior capsule opacification (PCO) requiring neodymium:YAG capsulotomy occurred in 31 eyes (28.70%); posterior synechias in 27 eyes (25.00%); cystoid macular edema (CME) in 23 eyes (21.30%); recurrent uveitis in 6 eyes (5.55%); and epiretinal membrane formation, glaucoma, and iris bombe in 5 eyes (4.63%) each.

CONCLUSIONS: Phacoemulsification with IOL implantation improved vision in most patients with coexisting cataract and uveitis. The main complications affecting visual outcomes were macular involvement, CME, PCO, and glaucoma.

Perylene indicated a higher D value and was homogeneously distrib

Perylene indicated a higher D value and was homogeneously distributed in the case of the OCA sample; we found that diffusivity and distribution of perylene in CA film were largely affected by the multistratification treatment. We also proved that the deeper the film depth was, the lower the diffusivity of perylene was, regardless

of the types of additives and films. The factors considered for the film-depth dependence of D were a gradual increase in the diffusion pathway for perylene caused by additive diffusion and the concentration dependence of the perylene D. (c) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 121: 1780-1787, 2011″
“The purpose of this study was to improve dissolution behavior of poorly water-soluble BAY 80-6946 nmr drugs by application of cyclodextrin in extrusion processes, which were melt extrusion process and wet extrusion process. Indomethacin (IM) was employed as a model drug. Extrudates containing IM and 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CyD) in 1:1 w/w ratio were manufactured by both melt extrusion process and wet extrusion process. In vitro drug release properties of IM from extrudates and physiochemical properties of extrudates were investigated. The dissolution rates of IM from extrudates manufactured

by melt extrusion and wet extrusion with HP-beta-CyD were significantly higher than that of the physical mixture of IM and HP-beta-CyD. In extrudate manufactured by melt extrusion, gamma-form of IM changed to amorphous AZD0530 concentration completely during melt extrusion due to heating above melting point of IM. On the other hand, in extrudate manufactured by wet extrusion, gamma-form of IM changed to amorphous partially due to interaction between IM and HP-beta-CyD and mechanical agitating force during process. Application of HP-beta-CyD in extrusion process is useful for the enhancement of dissolution rate for poorly water-soluble drugs.”
“A 21-year-old girl with an ischemic bowel developed portal and splenic vein thrombosis 3 weeks later, despite thromboprophylaxis

low-molecular-weight heparin. An extensive thrombophilia screen was negative and selleck chemical the only possible reason for her vascular occlusion was transient but severe eosinophilia. The role of transient eosinophilia in thrombosis is discussed in the light of other similar rare cases.”
“The feasibility of a gravure ink-containing nanoclays as an alternative oxygen (O(2)) barrier material to oriented polypropylene (OPP) film was investigated. The gravure ink was effectively dispersed through the clay using an ultrasonic homogenizer. Barrier properties of OPP films coated by the gravure ink-containing nanoclays were evaluated with respect to dispersion time, clay content, and different types of nanoclays (Cloisite Na(+), Cloisite 25A, and Cloisite 30B).

These results are particularly important considering the potentia

These results are particularly important considering the potential introduction of the Mn-containing gas additive, methyl cyclopentadienyl manganese tricarbonyl (MMT), in various countries around the world. In order to model this potentially

vulnerable population, we fed female rats fed either control (35 mg Fe/kg chow; 10 mg Mn/kg chow) or low iron/high-manganese (IDMn; 3.5 mg Fe/kg chow; 100 mg Mn/kg chow) diet, and examined whether these changes had any long-term behavioral effects on the animals’ spatial abilities, as tested by the Morris water maze (MWM). We also analyzed behavioral performance on auditory sensorimotor gating utilizing prepulse inhibition (PPI), which may be related to overall cognitive performance. Furthermore, brain and blood metal levels were assessed, as well as regional brain isoprostane production. We found that treated animals were slightly ID, with statistically PI3K inhibitors ic50 significant increases selleck inhibitor in both iron (Fe) and Mn in the hippocampus, but statistically significantly less Fe in the cerebellum. Additionally, isoprostane levels, markers of oxidative stress, were increased in the brain stem of IDMn animals. Although treated animals were indistinguishable from controls in the PPI experiments, they performed less well than controls in the MWM. Taken together, our data suggest that vulnerable ID populations

exposed to high levels of Mn may indeed be at risk of potentially dangerous alterations in brain metal levels which could also lead to behavioral deficits.”
“In this paper, we numerically investigate the highly directional beaming effect when a transverse magnetic plane wave passes through a metallic slit-bumps nanostructure by using the finite difference time domain method. We find that

the narrow bump with width w similar to 0.1 lambda(spp) can serve as a good light emitter and two arrays of narrow bumps with suitable separation can diffract the surface plasmons into highly directional collimated beams. The number and the direction of the beams can be controlled by adjusting the geometry parameters of selleck chemical the nanostructure. The structure with optimized parameters has practical applications as directional surface plasmon polaritons couplers in integrated photonic devices. (c) 2009 American Institute of Physics. [DOI: 10.1063/1.3139295]“
“Background: Implementation of guidance on assessment and management of psychosocial and supportive-care problems or needs will be successful only if consideration is given to existing skills, experience and expectations of staff and patients. This study examines the roles and responsibilities of staff, patients and families in relation to management of social difficulties and proposes a pathway for response.

Methods: A qualitative study was performed using staff and patient interviews. Seventeen doctors and 16 nurses were interviewed using patient scenarios and a support service questionnaire.

However, there is mounting

evidence that a number of kidn

However, there is mounting

evidence that a number of kidney allografts fail as a consequence of a rejection caused by antibodies specifically directed against major histocompatibility complex antigens, class I or II, of the recipient. A critical role in antibody-mediated rejection (AMR) is played by complement. A number of therapeutic attempts have been tried to prevent or treat AMR. The still open question is whether the antibodies we detect are those responsible for tissue damage or not.”
“Objective: Glycosphingolipids (GSLs) are ubiquitous membrane components that play a functional role in maintaining chondrocyte homeostasis. We investigated the potential role of gangliosides, one of the major components of GSLs, in osteoarthritis (OA) pathogenesis.

Design: selleckchem Both age-associated and instability-induced OA models were generated using GM3 synthase knockout (GM3.5(-/-)) mice. A cartilage degradation model and transiently GM3S-transfected chondrocytes were analyzed to evaluate the function of gangliosides in OA development. The amount Daporinad of each series of GSLs in chondrocytes after IL-1 alpha stimulation was profiled using mass spectrometry (MS).

Results: OA changes in GM3.5(-/-) mice were dramatically enhanced with aging compared

to those in wildtype (WT) mice. GM3.5(-/-) mice showed more severe instability-induced pathologic OA in vivo. Ganglioside deficiency also led to the induction of matrix metalloproteinase (MMP)-13 and ADAMTS-5 secretion and chondrocyte apoptosis in vitro. In contrast, transientGM3.5(-/-) transfection of chondrocytes suppressed MMP-13 and ADAMTS-5 expression after interleukin (IL)-1 alpha stimulation. GSL profiling revealed the presence of abundant gangliosides in chondrocytes after IL-1 alpha stimulation.

Conclusion: Gangliosides play a critical role in OA pathogenesis by regulating the expression of MMP-13 and ADAMTS-5 and chondrocyte apoptosis. Based on the obtained results, buy BTSA1 we propose that gangliosides are potential target molecules for the development of novel OA treatments. (C) 2013

Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.”
“Background: Home hemodialysis (HHD) has met with alternating fortunes. The present revival of interest is due to lower costs and more frequent/efficient treatments. HHD is underdeveloped, and a marketing approach may help in defining development strategies. The aim of this study was to systematically review the recent literature (2000-2010) according to a marketing approach, defining the potential of HHD according to the classical marketing items: market size, growth rate, profitability, trends, keys for success, needs for structures and distribution channels.

Methods: A Medline search was conducted for 2000-2010.

HOXA5-transfected OTC exhibited an aberrantly organized epitheliu

HOXA5-transfected OTC exhibited an aberrantly organized epithelium with significantly increased TUNEL staining as well as decreased PCNA and K5 staining, while expression of differentiation markers as K10, involucrin and filaggrin were somewhat enhanced. However, under in vivo environment in nude mice which had great paracrine regulatory mechanisms, the aberrant phenotype was ameliorated as shown by a more regular tissue organization and normal expression of PCNA and K5. Inversely, cells transfected with the homeodomain-deleted protein exhibited accelerated growth and produced

a more proliferative and better-orchestrated epidermis, as shown by well-expressed proliferation and differentiation markers.

Conclusions: HOXA5 can suppress keratinocytes growth and epidermal formation. It probably activated click here Selleckchem MDV3100 antagonist genes against growth factors release, which depends on its homeodomain. (C) 2012 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.”
“The aim of the study was to determine the clinical and biochemical characteristics of type 1 diabetes mellitus (DM) at presentation in children younger than 15 years in Croatia during a 9-year period, with special attention to diabetic ketoacidosis

(DKA) incidence. The registered data set comprised blood glucose, pH, serum bicarbonate levels, and clinical symptoms at disease manifestation. During the study period, 692 children were diagnosed with type 1 DM. Polydipsia (96.7%), polyuria (96.05%), and weight loss (82.7%) were the most frequent symptoms

anticipating disease detection. Enuresis was recorded in 11.55%. A total of 36.41% patients had DKA (pH<7.3) at disease onset. During the 9-year period, the percentage of children presenting with DKA at time of diagnosis decreased from 41.67% to 33.33% (z=1.68, p=0.046). A positive family history of DM, the only factor with an impact on the DKA incidence rate in our population, lowers the probability of the development of BYL719 mouse ketoacidosis. This study confirms the importance of the detection of the classic symptoms of polyuria, polydipsia, and weight loss in patients with new-onset type 1 DM. The percentage of patients with DKA at diabetes onset decreased during the observed period but is still high and includes one-third of all patients. This is why in every acutely ill child, especially at a younger age, one should evaluate the possibility of type 1 DM to avoid the development of ketoacidosis.”
“Background

Ectopic thyroid tissue is a rare abnormality that mainly occurs in the midline of the neck but also in other unusual locations. Because fine needle aspiration biopsy (FNAB) is a widely used procedure for evaluating bead and neck and other palpable masses, sampling of these lesions may be encountered.

Case

A 66-year-old woman presented with left lateral chest wall mass during follow-up fir chronic obstructive pulmonary disease, hypertension and diabetes mellitus.

098, p < 001) Conclusions: Our results demonstrate a strong

098, p < .001). Conclusions: Our results demonstrate a strong association of most of the MetSyn components with the SA dimension, but not with ASP2215 mouse the NA and PA scales.”
“Nilotinib (Tasigna) is

a BCR-ABL1 tyrosine kinase inhibitor approved for the treatment of patients with Philadelphia chromosome-positive chronic myeloid leukemia in chronic phase (CML-CP) who are newly diagnosed or intolerant of or resistant to imatinib. The 48-month follow-up data for patients with CML-CP treated with nilotinib after imatinib resistance or intolerance on an international phase II study were analyzed. Overall, 59% of patients achieved major cytogenetic response; 45% achieved complete cytogenetic response while on study. The estimated rate of overall survival (OS) and progression-free survival (PFS) at 48 months was 78% and 57%, respectively. Deeper levels of molecular responses at 3 and 6 months were highly positively correlated with long-term outcomes, including PFS and OS at 48 months. Of the

321 patients initially enrolled in the study, 98 (31%) were treated for at least 48 months. Discontinuations were selleck chemicals primarily due to disease progression (30%) or adverse events (21%). Nilotinib is safe and effective for long-term use in responding patients with CML-CP who are intolerant of or resistant to imatinib. Further significant improvements in therapy are required for patients who are resistant or intolerant to imatinib. Leukemia (2013) 27, 107-112; doi:10.1038/leu.2012.181″
“The aim of this study was to test and compare the effects of a within-subject design of repetitive

transcranial magnetic stimulation (rTMS) [coupled with sham transcranial direct current stimulation (tDCS)] and tDCS (coupled with sham rTMS) on the motor cortex excitability and also compare the results against sham tDCS/sham rTMS. We conducted a double-blinded, randomized, sham-controlled, cross-over trial. Eleven right-handed, healthy individuals (five women, mean age: 39.8 years, SD 13.4) received the three interventions (cross-over design) in a randomized order: (a) high-frequency (HF) rTMS (+sham tDCS), (b) anodal tDCS (+sham rTMS), and (c) sham stimulation (sham rTMS+sham tDCS). Cortical PSI-7977 ic50 excitability measurements [motor threshold, motor evoked potential (MEP), intracortical facilitation and inhibition, and transcallosal inhibition] and motor behavioral assessments were used as outcome measures. Between-group analysis of variance showed that MEP amplitude after HF rTMS was significantly higher than MEP amplitude after anodal tDCS (P=0.001). Post-hoc analysis showed a significant increase in MEP amplitude after HF rTMS (25.3%, P=0.036) and a significant decrease in MEP amplitude after anodal tDCS (-32.7%, P=0.001). There was a similar increase in motor function as indexed by Jebsen-Taylor Hand Function Test in the two active groups compared with sham stimulation.

(C) 2011 Elsevier Inc All rights reserved “
“Due to global

(C) 2011 Elsevier Inc. All rights reserved.”
“Due to global industrialization, the light cycle is shifting to longer daytime. Mounting evidence indicates that social developmental disorders may correlate with longer periods of daytime Gemcitabine clinical trial in childhood. However, the exact mechanisms of this link remain unclear. To examine the impact of longer day-time on psychosocial development, we developed a novel non-human primate model, using the common marmoset (Callithrix jacchus) reared under constant daylight from birth. Marmosets were reared individually by human nursing under constant light (LL) during varying periods in

juvenile development, and their behaviors were compared with those of normal day-night cycle (LD) marmosets by multivariate analysis based on principal component analysis (PCA). LL marmosets elicited egg-like calls (e-call) less in juvenile period, and displayed side-to-side shakes of the upper body with rapid head rotation through adulthood frequently. Based on the PCA, these

behaviors were interpreted as ‘alert’ or ‘hyperactive’ states. AZD6094 datasheet Additionally, behavioral development of marmosets reared under constant dark (DD) was markedly different from both LD and LL marmosets, suggesting the fundamental importance of daylight-dependent neuronal and endocrine processes and entrainment by a constant 24-hour light/dark cycle on psychosocial behavior development. (C) 2011 Elsevier Inc. All rights reserved.”
“The occurrence of urolithiasis in the United States has increased; however, information on long-term trends, including recurrence rates, is lacking. Here we describe national trends in rates of emergency department visits, use of imaging, and drug treatment, primarily using the National Hospital Ambulatory Medical Care Survey to describe trends and OICR-9429 supplier the National Health and Nutrition Examination Survey to determine the frequency of lifetime passage of kidney stones. Emergency department visit rates for urolithiasis increased from 178 to 340 visits per 100,000 individuals from 1992 to 2009. Increases

in visit rates were greater in women, Caucasians, and in those aged 25-44 years. The use of computed tomography in urolithiasis patients more than tripled, from 21 to 71%. Medical expulsive therapy was used in 14% of the patients with a urolithiasis diagnosis in 2007-2009. Among National Health and Nutrition Examination Survey participants who reported a history of kidney stones, 22.4% had passed three or more stones. Hence, emergency department urolithiasis visit rates have increased significantly, as has the use of computed tomography in the United States. Further research is necessary to determine whether recurrent stone formers receive unnecessary radiation exposure during diagnostic evaluation in the emergency department and allow development of corresponding evidence-based guidelines. Kidney International (2013) 83, 479-486; doi:10.1038/ki.2012.

Furthermore, the temporal sagittal system showed significant righ

Furthermore, the temporal sagittal system showed significant rightward asymmetry between patients and controls. These observations suggest a pattern of volume WM alterations associated with symptomatology in schizophrenia that may be related in part to predisposition

to schizophrenia. (C) 2010 Elsevier Ireland Ltd. All rights reserved.”
“Liquorice extract was reported to have nootropic and/or antiamnestic effects. Prepulse inhibition (PPI) of startle response is a multimodal, cross-species phenomenon used as a measure of sensorimotor gating. Previous studies PS-341 research buy indicated that liquorice/its constituents augmented mouse brain monoamine levels. Increased brain monoamines’ transmission was suggested to underlie PPI disruption. However, the effect of antiamnestic dose(s) of the extract on PPI has not been investigated despite the coexistence of impaired memory and PPI deficit in some neurological disorders. The effect of administration of the antiamnestic dose

of the extract (150 mg/kg for 7 days) was tested on PPI of acoustic startle response in mice. It resulted in PPI disruption and therefore its effect on monoamines’ levels was investigated Epacadostat purchase in a number of mouse brain areas involved in PPI response mediation. Results demonstrated that the extract antiamnestic dose augmented cortical, hippocampal and striatal monoamine levels. It was therefore concluded that liquorice extract (150 mg/kg)-induced PPI deficit was mediated through augmenting monoaminergic transmission. in the cortex, hippocampus and striatum. These findings can be further investigated in experimental models for autism, psychosis and Huntington’s disease to decide the safety of using liquorice extract

in ameliorating memory disturbance in disorders manifesting PPI deficit. LGX818 supplier (C) 2013 Elsevier Ireland Ltd. All rights reserved.”
“In this article, we present our first attempt at combining an elemental theory designed to model representation development in an associative system (based on McLaren, Kaye, & Mackintosh, 1989) with a configural theory that models associative learning and memory (McLaren, 1993). After considering the possible advantages of such a combination (and some possible pitfalls), we offer a hybrid model that allows both components to produce the phenomena that they are capable of without introducing unwanted interactions. We then successfully apply the model to a range of phenomena, including latent inhibition, perceptual learning, the Espinet effect, and first- and second-order retrospective revaluation. In some cases, we present new data for comparison with our model’s predictions.

We then compared the results from the calculators in terms of mea

We then compared the results from the calculators in terms of mean predicted resolution probability and number of cases deemed likely to resolve at various cutoff probabilities.

Results: Mean predicted resolution probabilities were 41% and 36% (range 31% to 41%) for the 2 academic affiliated https://www.selleckchem.com/products/dorsomorphin-2hcl.html calculators and 33% for the industry affiliated calculator (p = 0.02). For some patients the calculators produced markedly

different probabilities of spontaneous resolution, in some instances ranging from 24% to 89% for the same patient. At thresholds greater than 5%, 10% and 25% probability of spontaneous resolution the calculators differed significantly regarding whether cases would resolve (all p <0.0001).

Conclusions: Predicted probabilities of spontaneous resolution of vesicoureteral reflux differ significantly among Internet based calculators. For certain patients, particularly those with a lower probability of spontaneous resolution, these differences can significantly influence clinical decision making.”
“BACKGROUND

The

long-term effects of sibutramine treatment on the rates of cardiovascular events and cardiovascular death among subjects at high cardiovascular risk have not been established.

METHODS

We enrolled in our study 10,744 overweight or obese subjects, 55 years of age or older, with preexisting cardiovascular disease, type 2 diabetes mellitus, or both to assess the cardiovascular consequences of weight management with and without sibutramine in subjects at high risk for cardiovascular events. All Saracatinib mw selleck screening library the subjects received sibutramine in addition to participating in a weight-management program during a 6-week, single-blind, lead-in period, after which 9804 subjects underwent random assignment in a double-blind fashion to sibutramine (4906 subjects) or placebo (4898 subjects). The primary end point was the time from randomization to the first occurrence of a primary outcome event (nonfatal myocardial infarction, nonfatal stroke, resuscitation after cardiac arrest, or cardiovascular death).

RESULTS

The mean duration of treatment was 3.4 years. The mean weight loss

during the lead-in period was 2.6 kg; after randomization, the subjects in the sibutramine group achieved and maintained further weight reduction (mean, 1.7 kg). The mean blood pressure decreased in both groups, with greater reductions in the placebo group than in the sibutramine group (mean difference, 1.2/1.4 mm Hg). The risk of a primary outcome event was 11.4% in the sibutramine group as compared with 10.0% in the placebo group (hazard ratio, 1.16; 95% confidence interval [CI], 1.03 to 1.31; P = 0.02). The rates of nonfatal myocardial infarction and nonfatal stroke were 4.1% and 2.6% in the sibutramine group and 3.2% and 1.9% in the placebo group, respectively (hazard ratio for nonfatal myocardial infarction, 1.28; 95% CI, 1.04 to 1.57; P = 0.02; hazard ratio for nonfatal stroke, 1.36; 95% CI, 1.04 to 1.77; P = 0.03).

Furthermore, following infection with bovine rotavirus (BRV) over

Furthermore, following infection with bovine rotavirus (BRV) over 80% of cells in the ileal and jejunal cultures contained viral protein at 16 h post-infection. The intestinal epithelial cell cultures also contained fibroblasts so a jejunal fibroblast culture was established DihydrotestosteroneDHT solubility dmso and infected with BRV. Viral protein was detected in jejunal fibroblasts but viral-induced cytopathology was delayed in fibroblast cultures when compared to epithelial cell cultures. This study describes an effective protocol for culturing bovine epithelial

cells from fetal intestine and confirmed that the epithelial cells were susceptible to BRV infection. Real and jejunal cultures displayed limited growth following continuous passage but early passage epithelial cells provide competent target cells for studying host cell responses to an enteric viral pathogen. (C) 2007 Elsevier B.V. All rights reserved.”
“Subcortical projection from layer 5 neurons is the major cortical BAY 11-7082 supplier output. A transcription factor, Otx1, which is expressed in the layer 5 subcortical projection neurons in the visual cortex, was reported to be responsible for the establishment of

visual area-specific layer 5 subcortical projections by inducing the sensorimotor cortex to adopt a visual cortex identity. However, we here demonstrate that the area of corticospinal neurons shifted caudo-medially in the Otx1-null mice of which cortex is 9 tenths in size compared with that of the wild-type littermates, while the whole visual cortex did not convert to the sensorimotor cortex in the absence of Otx1. This suggests that Otx1 is not crucial for FRAX597 research buy the development of visual cortex identity but for the determination of the proportion of cortical areas to the whole neocortex. (C) 2008 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.”
“Varicella Zoster Virus (VZV) is the causative agent for both varicella (chicken pox) and herpes zoster (shingles).

As a member of the human herpesvirus family, VZV contains a large DNA genome, encoding 70 unique open reading frames (ORFs). The functions of the majority of these ORFs remain unknown. Recently, the full-length VZV (P-Oka strain) genome was cloned as a VZV bacteria artificial chromosome (BAC) and additionally a firefly luciferase cassette was inserted to generate a novel luciferase VZV BAC. In this study, a highly efficient protocol has been developed exploiting the new luciferase VZV BAC system to rapidly isolate and characterize VZV ORF deletion mutants by growth curve analysis in cell culture. Published by Elsevier B.V.”
“The spontaneous recovery of sensory, motor, and cognitive functions after stroke is thought to be mediated primarily through the reorganization and rewiring of surviving brain circuits.