The first methodology this website is the ISS which is based on a first step of

thin film fabrication, and then a second step where the synthesis of silver nanoparticles into the films is performed. The second methodology is the LbL-E deposition technique which follows a different order because firstly silver nanoparticles of a specific shape are synthesized, and then their incorporation into thin films using the LbL assembly is performed. Although both processes use the same reagents, remarkable differences related to the size, distribution, or maximal wavelength position of the LSPR band have been observed. Additionally, a thermal post-treatment was performed to fabricate stable hydrogel films with a better chemical stability via cross-link of the polymeric chains. This comparative study can be useful to the further design of advanced hybrid coatings based on metallic nanoparticles and

polymeric materials. Methods Materials Poly(allylamine hydrochloride) (Mw 56,000), poly(acrylic acid, sodium salt) 35 wt.% solution in water (PAA) (Mw 15,000), silver nitrate solution (> 99% titration, 0.1 N AgNO3), and dimethylamine Fer-1 order borane complex (DMAB) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used without any further purification. Aqueous solutions of 0.01 M of both PAH and PAA were prepared using ultrapure deionized water (18.2 MΩ) and adjusted to pH values 7.0 and 9.0 by the addition of a few drops of HCl or NaOH 1 M. Fabrication of the NF-��B inhibitor thin films All the thin films have been fabricated using a 3-axis

Cartesian robot from Nadetech Innovations SL (Sarriguren, Spain). The LbL assembly was performed by sequentially exposing the glass slides to cationic and anionic polyelectrolytes with an immersion time of 2 min. A rinsing step in deionized water was performed between Edoxaban the two polyelectrolyte baths. The combination of a cationic monolayer with an anionic monolayer is called bilayer. More details of the LbL assembly can be found elsewhere [37]. In situ synthesis of the silver nanoparticles This process starts with a first step of a multilayer coating fabrication using the LbL assembly of cationic (PAH) and anionic (PAA) polyelectrolytes. A second step is where the ISS of the AgNPs into the polymeric coating was carried out. The polymeric thin films are firstly immersed in an aqueous solution of silver nitrate (AgNO3 0.01 N) at room temperature for 5 min, removed, and rinsed with ultrapure water. Then, once the silver ions have been incorporated into films via ion exchange, a further in situ chemical reduction of the silver cations (Ag+) to silver nanoparticles (Ag0) was performed at room temperature. The films are immersed in an aqueous solution of dimethylamine borane complex (DMAB 0.01 N) for 5 min, removed, and rinsed with ultrapure water.