g–i Asymmetrical, 1-septate reddish-brown ascospores. Scale bars: a = 1 mm, b = 100 μm, c = 50 μm, d–i = 20 μm Ascomata 350–530 μm high × 550–700 μm diam., solitary, densely scattered, or in small groups

of 2–4, immersed, with a protruding papilla, 110–160 μm high, 160–250 μm diam., globose or subglobose, black, covered with white crystalline material which becomes hyaline and gel-like in water, ostiolate (Fig. 29a and b). Peridium 18–25 μm thick laterally (excluding the rim), up to 35 μm thick at the apex, thinner at the base, 1-layered, composed of small pale brown thin-walled CX-4945 price cells of textura prismatica, cells 5–12 × 3–5 μm diam., cell wall up to 1 μm thick, apex cells smaller and walls thicker (Fig. 29b). Hamathecium of dense, long pseudoparaphyses,

2–3 μm broad, branching and anastomosing between and above the asci. Asci 150–190(−230) × 12.5–15 μm (\( \barx = 172.5 \times 13.4\mu m \), n = 10), (6-)8-spored, rarely 4-spored, bitunicate, fissitunicate, cylindrical, with a furcate pedicel which is up to 40 μm long, ocular chamber not observed (Fig. 29c, d and e). Ascospores 19–22.5 × 10–12 μm (\( \barx = 20.2 \times 11.4\mu m \), n = 10), uniseriate to obliquely uniseriate and partially overlapping, broadly ellipsoid with broadly to narrowly rounded ends, reddish brown, 1-septate, constricted at septum, asymmetric with a larger upper cell, thick-walled, possibly distoseptate (Fig. 29f, g and h). Anamorph: Aplosporella-like (for detailed description see Rossman et al. 1999). Conidiomata globose, ca. 300 μm diam. Conidia holoblastic, broadly fusoid,

13–15 × 7–10 μm, Selleckchem MM-102 dark brown, finely spinulose (Rossman et al. 1999). Material examined: ARGENTINA, Buenos Aires, Tuyu, on Celtis tala Gill., Jan. 1881, leg. det. C. Spegazzini (NY, isotype; LPS, holotype). Notes Morphology When established Dubitatio, Spegazzini (1881) considered it as intermediate between Sphaeriaceae and Nectriaceae as has been mentioned by Rossman et al. (1999). Müller and von Arx (1962) Dichloromethane dehalogenase treated Dubitatio as a synonym of Passerinula, while the differences of ascomata and ascospores could easily distinguish these two genera (Rossman et al. 1999). After checking the type specimen, Dubitatio was assigned to Dothideomycetes, and considered closely related to Dothivalsaria in the Massariaceae (Barr 1979b, 1987b). Dubitatio chondrospora was assigned to Pseudomassaria (as P. chondrospora (Ces.) Jacz.) (Barr 1964; Müller and von Arx 1962). Phylogenetic study None. Concluding remarks The black ascomata with white crystalline covering and central white ostiolar region as well as the asymmetrical reddish brown ascospores are striking characters of Dubitatio dubitationum. The genus cannot be assigned to any family with certainty based on morphological characters and fresh EX527 collections are needed for sequencing. Entodesmium Reiss, Hedwigia 1: 28 (1854). (Phaeosphaeriaceae) Generic description Habitat terrestrial, saprobic (or parasitic?).