gattii strains for additional assay validation Culture collection ID Geographic origin Sample type MLST Year of isolation B4501 Australia Human VGI unknown B4503 Australia Human VGI unknown B4504 Australia Human VGI unknown B4516 Australia Human VGI unknown B5765 India Environmental VGI unknown B9018 California Human
VGI 2011 B9019 New Mexico Human VGI 2011 B9021 Rhode Island Human VGI 2011 B9142 Georgia Human PR-171 manufacturer VGI 2011 B9149 California Human VGI 2011 B8508 Oregon Human VGIIa 2009 B8512 Oregon Alpaca VGIIa 2009 B8558 Washington Human VGIIa 2010 B8561 Washington Human VGIIa 2010 B8563 Washington Human VGIIa 2010 B8567 Washington Dog VGIIa 2010 B8854 Washington Human VGIIa 2010 B8889 Oregon Environmental VGIIa 2010 B9077 Washington
Environmental VGIIa 2011 B9296 British Columbia Environmental VGIIa 2011 B8211 Oregon Selleckchem SB431542 Human VGIIb 2009 B8966 Oregon Horse VGIIb 2010 B9076 Washington Environmental VGIIb 2011 B9157 Washington Horse VGIIb 2011 B9170 Washington Porpoise VGIIb 2011 B9234 Washington Cat VGIIb 2011 B9290 British Columbia Cat VGIIb 2011 B9241 Oregon Human VGIIb 2011 B9428 Washington Cat VGIIb 2012 B9159 Washington Sheep VGIIc 2011 B9227
Oregon Cat VGIIc 2011 B9235 Oregon Human VGIIc 2011 B9244 Oregon Human VGIIc 2011 B9245 Oregon Human VGIIc 2011 B9295 British Columbia Environmental VGIIc 2011 B9302 Oregon Environmental VGIIc 2011 B9374 Oregon Human VGIIc 2011 B8965 New Mexico Human VGIII 2010 B9148 California Human VGIII 2011 B9151 Michigan Human VGIII 2011 B9163 New Mexico Human VGIII 2011 B9237 New Mexico Cat VGIII 2011 B9372 Cediranib (AZD2171) California Cow VGIII 2011 B9422 Oregon Cat VGIII 2012 B9430 Alaska Cat VGIII 2012 B7238 Botswana Human VGIV 2005 B7240 Botswana Human VGIV 2005 B7243 Botswana Human VGIV 2005 B7247 Botswana Human VGIV 2005 B7249 Botswana Human VGIV 2005 B7260 Botswana Human VGIV 2006 B7262 Botswana Human VGIV 2006 B7263 Botswana Human VGIV 2006 B7264 Botswana Human VGIV 2006 B7265 Botswana Human VGIV 2006 Isolate culturing and DNA extraction Isolates were grown on Yeast Peptone Glucose (YPD) agar plus 0.5% NaCl at 37°C for 24 hours; and DNA was prepared using an UltraClean DNA Isolation Kit as described by the manufacturer, with some modifications (MO BIO Laboratories, Carlsbad, CA). Briefly, ~0.