We tested its activity in biological fluids and in vivo using a mouse model of Salmonella typhimurium infection,
and examined the effect of cathelicidin-BF on Salmonella invasion to epithelial cells. In addition, the biodistribution of cathelicidin-BF was evaluated by using in vivo optical imaging. The results revealed that Nutlin-3 cathelicidin-BF was unstable in gastrointestinal tract, but retained substantially active in murine serum. Cathelicidin-BF attenuated the clinical symptoms of Salmonella infected-mice, significantly reduced the number of internalized Salmonella and attenuated Salmonella-induced decreases in TER in epithelial cells. Our results provide a first indication for the potential of cathelicidin-BF as a novel therapeutic option for salmonellosis. (C) 2015 Elsevier GmbH. All rights reserved.”
“We enlarged the uniconazole (UNI) molecule to find a specific inhibitor of abscisic acid (ABA) 8′-hydroxylase, and synthesized various UNI derivatives that were substituted with hydrophilic and hydrophobic groups at the 4-chlorine of the phenyl group of UNI using click chemistry. Considering its potency in ABA 8′-hydroxylase inhibition, its small effect on seedling growth, and its ease of application, UT4, the UNI derivative containing
the C(4) alkyltriazole, was the best candidate for a highly selective inhibitor of ABA 8′-hydroxylase. (C) 2009 Elsevier Ltd. All rights reserved.”
“Objectives To assess the expression of early cardiac genes, implicated in the hypertrophic growth response of the phosphatase inhibitor adult heart, in peripheral blood mononuclear cells in patients with essential hypertension and its relationship to ambulatory blood pressure monitoring (ABPM) parameters and to echocardiographic left ventricular mass.\n\nMethods Twenty-four-hour ABPM, echocardiography and blood sampling were performed in 62 untreated participants with essential hypertension. Blood samples from 38
healthy individuals were included for comparison. Peripheral blood mononuclear cells (PBMCs) HDAC inhibition were isolated and gene transcript levels were determined by quantitative real-time reverse transcription PCR.\n\nResults Myocardin (3.92 +/- 0.68 versus 2.09 +/- 0.67, P < 0.001), GATA4 (3.48 +/- 0.68 versus 0.32 +/- 0.08, P < 0.001) and Nkx2.5 (208.91 +/- 35.01 versus 129.75 +/- 49.70, P < 0.001) were upregulated in hypertensive patients compared with controls. In hypertensive patients, transcript levels of myocardin (r=0.698, P < 0.001) and GATA4 (r=0.374, P=0.003) showed significantpositive correlations with 24-h systolic blood pressure (BP) as well as with mean BP, (r=0.626, P < 0.001) and (r=0.340, P=0.007), respectively. A significant positive correlation between myocardin and 24-h pulse pressure (r=0.467, P < 0.001) was also observed. Myocardin (r=S0.606, P < 0.001) and GATA4 (r=S0.453, P < 0.