The study protocol was approved by the Ethical Committee of Central Finland Health Care District. Written informed consents were obtained GSK1210151A from all subjects prior to the assessments. The study subjects were recruited from Jyväskylä Central Finland Health District/Health Promotion Hospital initiative program. The study was conducted from May to November in 2009. One hundred and sixty-one women (all hospital staff) responded to our invitation. Sample size was estimated for different purposes on the primary outcome of the study results (such as physical fitness measured by walking test and maximum oxygen uptake (VO2max), heart rate

(HR) variability, and insulin resistance). All the sample sizes will have 85% power to detect differences with p < 0.05 two-sided significance level. Taking into account the compliance and drop-out (10%), the total number of subjects was estimated to meet each specific aims. One hundred met the screening criteria and were invited by phone or e-mail to participate in baseline measurements. A physician examined the physical condition of the subjects and ensured that subjects meet the inclusion criteria: 20–50 years old pre-menopausal woman with

a body mass index (BMI) between 25 and 38 kg/m2 who participated regular exercise ≤2 times/week and ≤45 min/time, and had no serious cardiovascular or musculoskeletal problems, no diagnosed type I diabetes, and had not lost Dolutegravir molecular weight or gained more than 5 kg in weight during the previous 6 months. The eligible participants were randomized into parallel exercise (EX) or diet intervention (DI) groups after being enrolled to the study and the qualification being checked. A computer program (http://www.random.org/) was used to generate the random number by blocks (50 numbers in each block). The randomized number was assigned to the subjects according Oxalosuccinic acid to the order of enrollment. The randomization was done by a researcher that was blinded during the trial and was unblinded at the conclusion of the trial. All

main investigators in the project were also blinded until the end of the intervention. The flow chart of the study participants is given in Fig. 1. Ninety women started intervention (n = 45 in the exercise group (EX) and n = 45 in the diet group (DI)). Eighty-three women completed the 6-week intervention and had follow-up measurements (39 in the EX and 44 in the DI). The study was registered in International Standard Randomized Controlled Trial Number Register (ISRCTN87529813). Full trial protocol is available online (http://www.controlled-trials.com/ISRCTN87529813/87529813). Background information including medical history and current health status was collected via self-administered questionnaires.