The MIC value was read where the growth inhibition ellipse intersected the antibiotic gradient concentration. The susceptibility test was controlled by the quality control organism,

Escherichia coli ATCC 25922. The test bacteria were categorised as susceptible or resistant as per published criteria [10]. Detection of genes mediating ESBL production All DEC strains were screened for ESBL production by the Etest ESBL method using Baf-A1 cell line both ceftazidime/ceftazidime combined with clavulanic acid and cefotaxime/cefotaxime combined with clavulanic acid acid strips (AB Biodisk) as described previously [11]. The three common β-lactamase-encoding genes, bla TEM, bla SHV and bla CTX-Mand the insertion sequence mobilizing the bla CTX-M gene, ISEcp1 were detected by PCR assays

as described previously [11]. The expected amplicon sizes were 971 bp (bla TEM), 798 bp (bla SHV), 543 bp (bla CTX-M) and 527 bp (ISEcp). The PCR products of bla CTX-M and ISEcp genes were sequenced and compared with the sequences in the public data bank by BLAST (Basic Local Alignment Search Tool) algorithm to determine their types. Statistics The significance of the difference in the prevalence of pathogens between patients and controls was calculated by Chi square test. A P value ≤ 0.05 was considered significant. Results The age stratification of children with diarrhoea and control children from AH and FH is shown in Table 1. The majority of the patients and controls were ≤ 2 years of age. The detection of DEC from case-control study of children in AH and FH is shown

in Table 2. A total of 85 (15.8%) diarrhoeal selleck chemicals children harboured a DEC. Among these 85 children were 2 children with dual infections: 1 had an EPEC and EAEC and the other ETEC and EIEC. The prevalence was greatest Thiamet G for EPEC among patients. Comparison of prevalence of EPEC between patients and controls did not show statistically significant difference. Of the 45 patients positive for EPEC, 21(6.01%) were up to two years of age. Of the 8 control children positive for EPEC, 7 (7.95%) were up to two years of age. There was no significant difference in the prevalence of EPEC between patients and controls up to 2 years of age (P = 0.68). Only 2 patients harboured typical EPEC (positive for both attaching and effacing gene and bundle-forming pilus gene). The other 43 patients and all 8 controls positive for EPEC harboured atypical EPEC isolates (positive for attaching and effacing gene only). The other SB431542 ic50 categories of DEC were present in a small number of patients and not in controls. Table 1 Age strata of 537 diarrhoeal children and 113 control children from Al-Adan and Al-Farwaniya hospitals, Kuwait Age (months) strata Number of diarrhoeal children Number of control children 0–12 250 69 13–24 99 19 25–36 88 8 37–48 60 8 49–60 40 9 Table 2 Detection of diarrhoeagenic E.