1H NMR (DMSO-d 6) δ (ppm): 7.60 (t, 3H, CHarom., J = 3.6 Hz), 7.56–7.55 (m, 1H, CHarom.), 7.53–7.48 (m, 2H, CHarom.), 7.47–7.44 (m, 6H, CHarom.), 7.40–7.31 (m, 3H, CHarom.), 7.20–7.08 (m, 2H, CHarom.), 6.23 (d, 1H, CHarom., J = 7.8 Hz), 3.51–3.28 (m, 6H, CH2), 3.19–3.07 (m, 6H, CH2), 1.70–1.68

(m, 2H, CH2), 1.58–1.53 (m, 6H, CH2). 13C NMR (CDCl3) δ (ppm): 190.30, 165.71, 165.49, 149.83, 148.79, 141.26, 137.44, 135.86, 134.92, 134.77, 134.51, 133.34 (2C), 132.58 (2C), 130.93 (2C), 129.81 (2C), 129.79 (2C), 128.73 (3C), 128.52 (3C), 128.39 (2C), 127.04 (2C), 124.82, 123.17, 58.14, 58.07, 52.58, 52.47, 35.97, 34.06, 29.74, 26.11. ESI MS: m/z = 652.4 [M+H]+ (100 %). Synthesis of 2-4-[4-(2-metoxyphenyl)piperazin-1-yl]butyl-4,10-diphenyl-1H,2H,3H,5H-indeno[1,2-f]isoindole-1,3,5-trione Fer-1 (19) Yield: 79 %, m.p. 245–246 °C. 1H NMR (DMSO-d 6) δ (ppm): 7.61 (t, 3H, CHarom., J = 3.6 Hz), 7.56–7.44 (m, 8H, CHarom.), 7.41–7.31 (m, 2H, CHarom.), 7.05–6.87 (m, 4H, CHarom.), 6.23 (d, 1H, CHarom., J = 6.9 Hz), 3.79 (s, 3H, OCH3), 3.47–3.44 Idasanutlin research buy (m, 6H, CH2), 3.07–2.97 (m, 6H, CH2), 1.69–1.67 (m, 2H, CH2), 1.59–1.52 (m, 2H, CH2). 13C NMR (CDCl3) δ (ppm):

192.35, 165.07, 164.79, 149.81, 148.96, 141.13, 137.77, 135.42, 134.37, 134.26, 134.08, 133.11 (2C), 132.66 (2C), 130.72 (3C), 129.86, 129.72 (2C), 128.91 (3C), 128.54 (2C), 128.21 (3C), 127.75 (2C), 124.11, 123.59, 62.00, 58.84, 58.71, 52.97, 52.84, 35.06, 34.26, 29.59, 26.91. ESI MS: m/z = 648.3 [M+H]+ (100 %). 3-4-[4-(2-Metoxyphenyl)piperazin-1-yl]butyl3-azatricyclo[7.3.1.05,13]trideca-(12),5,7,9(13),10-pentaene-2,4-dione (20) was obtained according to method presented previously (Hackling et al., 2003) Yield: 63 %, m.p. 279–282 °C. 1H NMR (DMSO-d 6) δ (ppm): 8.59–8.48 (d, 2H, CHarom., J = 8.1 Hz), 8.11 (d, 2H, CHarom., J = 7.8 Hz), 7.64 (t, 2H, CHarom., J = 7.6 Hz), 7.08–6.76 (m, 4H, CHarom.) 4.56–4.17 (m, STK38 2H, CH2), 3.87 (s, 3H, OCH3), 3,41–2.98 (m, 5H, CH2), 2.93–2.32

(m, 5H, CH2), 2.04–1.42 (m, 4H, CH2). 13C NMR (CDCl3) δ (ppm): 165.72, 159.08, 158.97, 140.62, 134.22, 134.17, 134.09, 133.74, 132.25, 130.14, 129.64, 129.53, 128.47, 128.38, 128.09, 127.48, 124.02, 123.61, 61.13, 60.95, 57.53, 51.27, 51.13, 41.37, 41.29, 26.96, 26.87. ESI MS: m/z = 344.6 [M+H]+ (100 %). Biological assays Cell-based assays Cell-based assays were performed at Dipartimento di Scienze e Tecnologie Biomediche, Università di Cagliari, Monserrato, Italy. Test compounds Compounds were dissolved in DMSO at 100 mM and then diluted in culture medium. Cells and viruses Cell line and viruses were purchased from the American Type Culture Collection (ATCC).