Nonetheless, the predictive role of liver enzymes has never been confirmed in a

real-life context, where patients are not tested per-protocol and results are obtained from labs with different analyzers. We aimed to verify HM781-36B concentration the association between baseline ALT, GGT and AST/ALT ratio, the latter as a proxy of liver disease evolution, and the incidence of DM, stroke and coronary heart disease (CHD), in a large real-life population. Patients and Methods. Subjects who underwent routine blood tests including AST, ALT and GGT between 2000 and 2005 were extracted from a validated software employed by 120 general practitioners in the area of Naples (Italy), in charge of about 170.000 subjects. Incident DM, stroke and CHD were registered after a median follow-up time of

102 months (8.5 years). After exclusion criteria (known liver disease, HBsAg+, HCVAb+, age<20), data from 16.689 subjects were analyzed. Results. Mean age of the study population was 62.3 +/- 17.7, male/female 43.8/56.2%. Cumulative incident DM, stroke and CHD were respectively 5.1%, 1.2% and 4.6%. In multivariate-adjusted analysis, ALT was associated with incident DM (OR 1.17; CI 1.06-1.29; p=0.002), but not with stroke and CHD. GGT was associated with incident DM (OR 1.32; CI 1.19-1.46; p<0.001), and stroke (OR 1.25; CI 1.05-1.49; p=0.009), but not with CHD, while AST/ALT ratio was not associated with any outcome. DM was Navitoclax diagnosed in 3.2%, 5.2% and 6.9% of subjects with baseline GGT in the lower, medium and upper tertile, respectively (p=0.02). Conclusion. Except for GGT and incident stroke, our study, the first carried out in a real-life setting,

does not support an association between baseline liver enzymes and the occurrence of vascular events, while confirms an independent predictive role of both ALT and GGT levels for incident DM. These results add to the accumulating evidence that the liver is a strong contributor to insulin resistance rather than a simple target of dysmetabolism. Disclosures: Antonio Picardi – Grant/Research Support: Schering-Plough, Roche, Schering-Plough, Roche, Schering-Plough, Roche, Schering-Plough, Roche; Speaking and Teaching: Bayer, Sclareol Bayer, Bayer, Bayer The following people have nothing to disclose: Antonio De Vincentis, Umberto Vespasiani Gentilucci, Gaetano Piccinocchi, Roberto Piccinocchi, Giovanni Galati, Paolo Gallo, Chiara Dell’Unto Polychlorinated biphenyls (PCBs) are organic environmental pollutants. In experimental studies, addition of PCB congener 153 to high-fat diet produced liver inflammation distinctive for nonalcoholic steatohepatitis (NASH), however no data were published concerning the distribution of PCB congeners in the blood of healthy controls and patients with NASH.