J Bacteriol 1996,178(6):1646–1654.PubMed 43. Hardason G: Methods for measuring biological nitrogen fixation in grain legumes. Plant and Soil 1993, 152:1–17.CrossRef 44. Charles TC, Finan TM: Analysis of a 1600-kilobase Rhizobium

meliloti megaplasmid using defined deletions generated in vivo. Genetics 1991, 127:5–20.PubMed 45. Sambrook JRD: Molecular Cloning: a laboratory manual. 3rd edition. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 2001. 46. Brinkmann E, Beckwith J: Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and ϕ 80 transducing lysates. J Mol Biol 1975, 96:307–316.CrossRef 47. Law J, Slepecky R: Assay of poly-3-hydroxybutyric acid. J Bacteriol 1961, 82:33–36.PubMed 48. Jensen H: Nitrogen www.selleckchem.com/TGF-beta.html fixation www.selleckchem.com/products/BI-2536.html in leguminous plants. I. General characters of root-nodule bacteria isolated from species of Medicago and Trifolium in Australia. Australia Proc Linn Soc NSW

1942, 66:98–108. 49. Venable JH, Coggeshall R: A Simplified Lead Citrate Stain for Use in Electron Microscopy. J Cell Biol 1965, 25:407–8. [0021–9525 (Print) Journal Article]PubMedCrossRef 50. Meade HM, Long SR, Ruvkun GB, Brown SE, Ausubel FM: Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn 5 mutagenesis. J Bacteriol 1982, 149:114–22. [0021–9193 (Print) Journal Article]PubMed 51. Hanahan D: Studies on transformation of Escherichia Cobimetinib in vitro coli with plasmids. J Mol Biol 1983,166(4):557–80. [0022–2836 (Print) Journal Article]PubMedCrossRef 52. Finan TM, Kunkel B, De Vos GF, Signer ER:

Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes. J Bacteriol 1986, 167:66–72. [0021–9193 (Print) Journal Article]PubMed 53. Schafer A, Tauch A, Jager W, Kalinowski J, Thierbach G, Puhler A: Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum . Gene 1994, 145:69–73.PubMedCrossRef 54. Jones JD, Gutterson N: An efficient mobilizable cosmid vector, pRK7813, and its use in a rapid method for marker exchange in Pseudomonas fluorescens strain HV37a. Gene 1987,61(3):299–306.PubMedCrossRef Authors’ contributions AZ generated the phaZ mutant. MAT performed the cloning reactions, carbon starvation assays, symbiosis assays, electron microscopy, supervised KNL and drafted the manuscript. KNL conducted carbon starvation assays. TCC constructed the pD82exoF::TnphoA vector. DC conducted the alkaline phosphatase assays and plant competition experiments. TCC and SRDC participated in experimental design and data analysis. All authors have read and approved the final manuscript.”
“Background buy GDC-0973 Trans-translation is a quality-control mechanism that is ubiquitous in bacteria and involves two activities [1–3].