In contrast, no significant correlations with egg quality were found for any of the genes in the unfertilized egg study ( Supplemental Fig. 3). For the 13 females in this study that had detectable

dcbld1 transcript expression in fertilized eggs, expression ranged from a relative quantity (RQ) of 1.0 (female 2) to an RQ of 14,659.4 (female 12) ( Fig. 3A; Supplemental Table 11). qPCR with unfertilized egg samples showed that dcbld1 transcript was detectable for 9 out of 13 females where expression ranged from an RQ of 1.0 (female 4) to an RQ of 917.2 (female 12) ( Fig. 4A; Supplemental Table 13). Interestingly, the two females with the highest dcbld1 transcript expression in both fertilized (RQ values of 13,776.8 and 14,659.4) and unfertilized eggs (RQ values of 782.1 and 917.2) were both from family B35 (females 11 and 12, respectively) (Figures 3Aand 4A; Supplemental Table 11 and Supplemental selleck Table 13). qPCR using fertilized egg samples

showed that aromatic-L-amino-acid-decarboxylase (synonym: dopa decarboxylase, ddc) transcript was detectable for all 15 females, with expression ranging from an RQ of 1.0 (female 15) to an RQ of 820.2 (female 12) ( Fig. 3B; Supplemental Table 11). In contrast, selleck chemicals qPCR with unfertilized egg samples showed that ddc transcript expression ranged from an RQ of 1.0 (female 1) to an RQ of 190.9 (female 11) ( Fig. 4B; Supplemental Table 13) in the 11 females in which it was detected. As seen for dcbld1, the two females with the highest ddc transcript expression in both fertilized eggs (RQ values of 769.9 and 820.2) and unfertilized eggs (RQ values of 190.9 and 188.3), with greater than 20-fold higher ddc expression than any other female, were both from family B35 (females 11 and 12, respectively) ( Figs. 3B and 4B; Supplemental Table 11 and Supplemental Table 13). In addition, for the other 3 families each represented by 2 females (B11, B33, and B84), fertilized egg ddc transcript expression levels for the 2 females of a given family were remarkably similar (e.g. RQ of 11.37 and 11.41 for females 1 and 13, respectively, in family B33) ( Fig. 3B; Supplemental Table 11). Carbohydrate However, when all females were considered, there was no correlation of either dcbld1

or ddc transcript expression and egg quality in either fertilized or unfertilized eggs ( Supplemental Figs. 2C,F and 3A,B). The acy3 transcript was detectable in the eggs from all females involved in the fertilized egg and unfertilized egg qPCR studies ( Figs. 3C and 4C). For both of these studies, female 2 had the lowest acy3 transcript expression (RQ of 1.0 for both studies, versus RQ ranges of 1.9–5.7 and 1.2–3.9 for other females in the fertilized egg and unfertilized egg studies, respectively; Supplemental Table 11 and Supplemental Table 13). These transcript expression results are intriguing in light of the fact that female 2 also had the lowest total mortality at both 3 and 7 dpf ( Fig. 1B,C), as well as the highest percent hatch (55.