Gene 1996,169(1):9–16.PubMedCrossRef 48. Brautaset T, Sekurova ON, Sletta H, Ellingsen TE, Strøm AR, Valla S, Zotchev SB: Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. Chem Biol 2000,7(6):395–403.PubMedCrossRef 49. He W, Lei J, Liu Y, Wang Y: The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin
biosynthesis in Streptomyces hygroscopicus 17997. Arch Microbiol 2008,189(5):501–510.PubMedCrossRef 50. Stragier P, Richaud F, Borne F, Patte JC: Regulation of diaminopimelate BAY 63-2521 cost decarboxylase synthesis in Escherichia coli. I. Identification of a lysR gene encoding an activator of the lysA gene. J Mol Biol 1983,168(2):307–320.PubMedCrossRef 51. Maddocks SE, Oyston PC: Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins. Microbiology 2008,154(Pt 12):3609–3623.PubMedCrossRef 52. Wilkinson CJ, Hughes-Thomas ZA, Martin
check details CJ, Bohm I, Mironenko T, Deacon M, Wheatcroft M, Wirtz G, Staunton J, Leadlay PF: Increasing the efficiency of heterologous promoters in actinomycetes. J Mol Microbiol Biotechnol 2002,4(4):417–426.PubMed 53. Martinez-Castro M, Barreiro C, Romero F, Fernandez-Chimeno RI, Martin JF: Streptomyces tacrolimicus sp. nov., a low producer of the immunosuppressant tacrolimus (FK506). Int J Syst Evol Microbiol 2011,61(Pt 5):1084–1088.PubMedCrossRef 54. Salehi-Najafabadi Z, Barreiro C, Martinez-Castro
M, Solera E, Martin JF: Characterisation of a gamma-butyrolactone receptor of Streptomyces tacrolimicus: effect on sporulation Acesulfame Potassium and tacrolimus biosynthesis. Appl Microbiol Biotechnol 2011,92(5):971–984.PubMedCrossRef 55. Chater KF, Chandra G: The use of the rare UUA codon to define “”expression space”" for genes involved in secondary metabolism, development and environmental adaptation in streptomyces. J Microbiol 2008,46(1):1–11.PubMedCrossRef 56. Chen D, Zhang Q, Cen P, Xu Z, Liu W: Improvement of FK506 production in Streptomyces tsukubaensis by genetic enhancement of the supply of unusual polyketide extender units via utilization of two distinct site-specific recombination systems. Appl Environ Microbiol 2012, 78:5093–5103.PubMedCrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions DG and MB carried out cloning, overexpression and gene disruption experiments, promoter activity studies, bioinformatic and data analysis, participated in experiment design and drafted the manuscript. VM participated in the selleck products initial set-up of the chalcone synthase reporter system and provided support with the experiments. JH performed the HPLC and data analysis. EK participated in the design of the genetically manipulated strains. TP provided analytical support. JSA performed the RT-PCR studies. MMC and CB performed RNA isolation. PM and GKopitar provided support with gene cluster sequence analysis and experiment design.