For this comparison, those values resulting in a probability other than zero are considered statistically distinct ( D’Suze and Sevcik, 2010). From the phase plot (ti,Qi), which represents Ts-DF venom (SWS) of Q = D − 1 plotted against the SWS of Q = D − 1 from Ts-MG venom (data not shown), and based

on the non-parametric Spearman rank correlation coefficient (rs, when ds = rs2), the coefficient of determination buy Omipalisib (ds) value obtained was 0.56 and rs (0.75) with P(rs = 0) of 3.19 × 10−20. Considering these values and that plotted points do not tend to cluster around a straight line, it is strengthened that venoms are different. MALDI-TOFMS analyses of Ts-DF and Ts-MG venom chromatographic fractions resulted in the detection of 171 and 174 components whose molecular masses ranged from m/z 1145.6 to 10,988.4 and 1196.8 to 16,457.5, respectively ( Fig. 5-A). Were observed in Ts-DF and Ts-MG venoms 114 corresponding molecular masses. Moreover, 54 (32.1%) were present only in Ts-DF venom, and 70 (38.0%) were exclusive for Ts-MG venom ( Fig. 5-B and Table 4). Ts-DF venom yielded a smaller number of peptides with molecular mass distributed between 6500 and 7500 Da than Ts-MG venom. On the other hand, 5001 to 5500 Da peptides were in higher number in Ts-DF venom than in Ts-MG one ( Figs. 5 and 6). T. serrulatus is considered the most important scorpion species for Public Health in Brazil

( Funasa, 2001 and Funasa, 2009). This is the first study to evaluate the toxicity of the venom of T. serrulatus from DF, Brazil, and the effects it provokes in vivo on murine learn more species. We demonstrated that the T. serrulatus venom from Distrito Federal (LD50 of 51.6 μg/mouse) is almost twice (1.98) less toxic than the T. serrulatus (MG)

venom (LD50 of 26.0 μg/mouse). Nishikawa et al. (1994) had previously shown for T. serrulatus the LD50 of 25.5 μg/mouse. The LD50 of the venom of T. serrulatus from Bahia, a northeastern Brazilian state also bordering MG, is 96.16 μg/mouse ( Silva et al., 2005a and Silva et al., 2005b), indicating the existence of differences in the venom of this species from different regions of Brazil. Factors such as milking and storage means of the venom, the route of venom administration on mice, and the observation time course of LD50 experiment could possibly result in different toxicities. However, as the experiments conducted here with both venoms Idoxuridine followed the same protocols, these factors were controlled, being the origin of scorpions the most acceptable hypothesis to reinforce the assertion for regional venom variation. The neurotoxins were the most important compounds of scorpion venom, acting on ion channels and resulting in an expressive release of acetylcholine, noradrenaline and adrenaline affecting both the sympathetic and parasympathetic systems, inducing physiological and behavioral changes (Henriques et al., 1968, Ismail, 1995, Dávila et al., 2002, Vasconcelos et al., 2005, Cupo et al.