Nucleic Acids Res 2005, 33:D230-D232.PubMedCrossRef 22. Kaplan CW, Kitts CL: Variation between observed and true Terminal Restriction Fragment length is dependent on true TRF length and purine content. J Microbiol Methods 2003, 54:121–125.PubMedCrossRef 23. Marsh TL: Culture-independent find more microbial community analysis with terminal restriction fragment length polymorphism. Methods Enzymol 2005, 397:308–329.PubMedCrossRef 24. Rusch DB, Halpern AL, Sutton G, Heidelberg KB, Williamson S, Yooseph S, Wu D, Eisen JA, Hoffman JM, Remington K, Beeson K, Tran

B, Smith H, Baden-Tillson H, Stewart C, Thorpe J, Freeman J, Andrews-Pfannkoch C, Venter JE, Li K, Kravitz S, Heidelberg JF, Utterback T, Rogers YH, Falcón LI, Souza V, Bonilla-Rosso G, Eguiarte LE, Karl DM, Sathyendranath S, Platt T, Bermingham E, Gallardo V, Tamayo-Castillo G, Ferrari MR, Strausberg RL, Nealson K, Friedman GSK461364 supplier R, Frazier M, Venter JC: The Sorcerer II Global Ocean Sampling expedition: northwest Atlantic through eastern tropical Pacific. PLoS Biol 2007, 5:398–431.CrossRef Authors’ contributions AFG wrote the

script and participated in the analysis and drafting of the manuscript. XM participated in the analysis and AB in the analysis and drafting of the manuscript. EOC coordinated the study, as well as participated in writing the manuscript. JMG conceived the study, and participated in its design and coordination. JMG was also involved in the analysis and interpretation of results and drafting of the manuscript. All authors read and approved the final manuscript.”
“Background Bacteria belonging to the phylum Planctomycetes

have revealed several remarkable features that set them apart from other bacteria. Their cryptic morphology led early microbiologists to mistake them for fungi, and the discovery of their cell compartmentalization, featuring membrane bounded Methane monooxygenase organelles, raised fundamental questions about the evolution of eukaryotes [1, 2]. Further, the unique anammox metabolism found in some planctomycetes has revolutionized the view of microbial nitrogen cycling [3]. The planctomycetes also possess cell walls without peptidoglycan, a characteristic that they share only with the obligate intracellular bacteria within Chlamydiae. In addition to the interest sparked by these unusual and fascinating features, planctomycetes have in later years attracted considerable attention because of their presence in a wide see more variety of environments on earth. By investigating bacterial communities using molecular methods (sequences coding for 16S rRNA), planctomycetes have been repeatedly detected in soil, sediments, marine and freshwater systems and in terrestrial hot springs to mention just a few (for a detailed review see [4]). However, their metabolic potential and function in these ecosystems is often unclear, as 16S rRNA gene sequence investigations only rarely give clues to ecological roles.