5% and 23 5% more biomass than WT under the WW, MD and SD treatme

5% and 23.5% more biomass than WT under the WW, MD and SD treatments. The transgenic plants showed higher grain yields than WT plants in both field and tank experiments and under the soil moisture treatments (Fig. 5). Antidiabetic Compound Library ic50 On average in the field experiment, transgenic plants had 15.5%, 22.4% and 21.0% higher grain yields than WT under WW, MD and SD treatments, respectively. In the tank experiment, transgenic plants had 16.7%, 18.0% and 19.0% higher grain yields than WT under WW, MD and SD treatments, respectively,

indicating an enhanced tolerance to drought in transgenic rice plants. Because the soil drought treatments were imposed beginning at 9 DPA, panicle number per area and spikelet number per panicle were not affected by the treatments (Table 5). In comparison with the WW treatments, the percentages of filled grains and grain weight decreased under both MD and SD treatments, with greater decreases under the SD than under the MD treatment. Under the same soil moisture, especially under the MD and SD treatments, both www.selleckchem.com/products/Roscovitine.html PPDK and PCK plants showed a greater percentage of filled grains than WT plants. Grain weight and harvest index varied with genotype and soil moisture treatment. Generally, the PCK plants exhibited higher grain weight and harvest index than WT plants under both MD and SD treatments (Table 5). Photosynthesis is fundamental to biomass

production, but sensitive to drought. Improving photosynthesis-related physiological traits is thought to be a useful approach to increase yield and drought tolerance [10], [32], [33] and [34]. Researchers worldwide have attempted to improve photosynthesis and crop yield by introducing C4 cycle in plants by transgenic approaches [4], [11], [12], [15], [35] and [36]. But there is a longstanding controversy as to whether an increase in leaf-level photosynthesis would increase

yield [37], [38], [39], [40] and [41]. In the present study, transgenic plants overexpressing key C4 enzymes not only had higher photosynthetic rates, but produced higher grain yields than WT plants. Given PAK5 that the WT cv. Kitaake and the two transgenic plants (PPDK and PCK) have the same genetic background and the only difference is in the expression level of several C4 key enzymes, our results provided direct evidence that increasing photosynthesis could result in a yield increase. The present results agree well with previous reports that transgenic rice plants show improved Pn and yield [4] and [10]. Transgenic plants showed Pn superior to the WT throughout the day and across the different grain filling stages (e.g. at 14 and 21 DPA). Ku et al. [4] observed that improved Pn was caused by increased stomatal conductance and increased internal CO2 concentration. In our study, higher leaf water content was observed for transgenic plants than for WT plants. The high leaf water content would contribute to increased stomatal conductance [42].

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