05), but was Protein Tyrosine Kinase inhibitor significantly higher than that of the PQ group on day 3 (P < 0.01). The significantly lowered TNF-α, IL-1β and IL-6 levels, and a significantly elevated IL-10 level were found in

the MP group compared with those of the BMSC group on day 1 after PQ poisoning (P < 0.01). The significantly lowered TNF-α, IL-1β and IL-6 levels, and a significantly elevated IL-10 level were found in the BMSC + MP group compared with those of the MP group and the BMSC group on day 7 Inhibitors,research,lifescience,medical (P < 0.01). (Table ​(Table22). Table 2 Plasma levels of cytokines ( ng/L, n = 6) Plasma levels of MDA and SOD Plasma levels of MDA in the PQ group were significantly elevated, and SOD levels were significantly decreased compared with those of the control

group (P < 0.01). On days 3–7 after PQ poisoning, the significantly lowered MDA levels, and a significantly elevated SOD level were found in the BMSC group, compared with those of the PQ group (P < 0.01).Plasma levels of MDA and SOD Inhibitors,research,lifescience,medical in the MP group were significantly lowered and elevated, respectively, on day 1 compared with those of the BMSC group (P < 0.01). MDA and SOD levels in the BMSC + MP group were significantly lowered and elevated, respectively, compared with those of BMSC group and MP group on days on days 3–7 (P < 0.01) (Table Inhibitors,research,lifescience,medical ​(Table33). Table 3 Plasma levels of MDA and SOD (mean ± SD, n = 6) Expression of NF-кB p65 in lung tissue NF-кB p65 expression in lung tissue from the PQ group was significantly elevated and peaked on day 3 after PQ poisoning, compared with that of the control group (P <

0.01). NF-кB p65 expression from the BMSC group was lowered on day 1 (P < 0.01), compared with that from the PQ group. NF-кB p65 expression in the MP group was significantly lowered on days 1–3, compared with Inhibitors,research,lifescience,medical that in the BMSC group (P < 0.01), but was similar to that from the BMSC group on day 7 (P > 0.05). The BMSC + MP group showed similar expressions of NF-кB p65, compared with those of the MP group on days 1–3 (P > 0.05), but were significantly lower than those of the BMSC group (P Inhibitors,research,lifescience,medical < 0.01). In addition, NF-кB p65 expressions in the BMSC + MP group on days 7-14 after PQ poisoning were lower than those in the BMSC group (P < 0.05) (Table ​(Table44). Table 4 Gray values for NF-кB p65 expression not in lung tissue (mean ± SD, n = 5) Discussion Alveolar cells actively uptake and accumulate PQ causing severe lung injury. The consensus of the poisoning mechanism is changes in redox potential. PQ activates NF-кB and promotes the expression and release of early inflammatory mediators. In addition, PQ produces large amounts of oxygen free radicals, induces the production of lipid peroxides and causes direct damage to major cell components [10-12]. Dinis-Oliveira et al [13] found that NF-кB is constantly elevated within 96 h after intraperitoneal injection of PQ, which is consistent with the findings of this study.