Localization

of liposomes in the tumor tissue was directly observed by fluorescence microscopy in live tumor-bearing mice. Conclusions Intratumoral injection is an effective method for liposome-mediated drug delivery into tumor tissues. The use of DOX-loaded DSPE-PEI cationic liposomes was found to result in significantly increased in vitro click here intracellular uptake compared with control liposomes. Notably, the conjugation of PEI to the liposomal membrane effectively improved the localization of drug-loaded liposomes at the tumor site through electrostatic interaction, which occurred in the tumor tissue of tumor-bearing mice treated with intratumorally injected liposomes. Our results demonstrate a promising approach to improve the intracellular uptake and localization effect of cationic liposomes. Although DSPE-PEI liposomes check details exhibit enhanced intracellular uptake, additional studies on the localization, injection route, and stability of these carriers is required for validation of their potential clinical application.

The cationic liposome delivery strategy presented here has considerable potential as a drug delivery platform for the treatment of a broad range of human diseases and can be adapted for other injection applications in various therapeutic fields. Acknowledgements This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2009–0078434) (BCS) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A2059167) (HDH). This work

was supported by Ulixertinib Basic Research Laboratory Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (No. 2013R1A4A1069575) (HDH). References 1. Allen TM, Cullis PR: Liposomal drug delivery systems: from concept to clinical applications. Adv Drug Deliv Rev 2012, 65:36–48.CrossRef 2. Safinya CR, Ewert KK: Materials chemistry: liposomes derived from molecular vases. Nature 2012, 489:372–374.CrossRef 3. Petersen AL, Hansen AE, Gabizon A, Andresen TL: Liposome imaging agents in personalized medicine. Adv Drug Deliv this website Rev 2012, 64:1417–1435.CrossRef 4. Moghimi SM, Szebeni J: Stealth liposomes and long circulating nanoparticles: critical issues in pharmacokinetics, opsonization and protein-binding properties. Prog Lipid Res 2003, 42:463–478.CrossRef 5. Drummond DC, Meyer O, Hong K, Kirpotin DB, Papahadjopoulos D: Optimizing liposomes for delivery of chemotherapeutic agents to solid tumors. Pharmacol Rev 1999, 51:691–743. 6. Jung SH, Kim SK, Kim EH, Cho SH, Jeong KS, Seong H, Shin BC: Increased stability in plasma and enhanced cellular uptake of thermally denatured albumin-coated liposomes. Colloids Surf B Biointerfaces 2010, 76:434–440.CrossRef 7. Han HD, Mora EM, Roh JW, Nishimura M, Lee SJ, Stone RL, Bar-Eli M, Lopez-Berestein G, Sood AK: Chitosan hydrogel for localized gene silencing.