6%), unnamed cultivable species (5.9%) and non-cultivable or uncultured phylotypes

(3.8%) and the sequences with <98% identity are unclassified species (11.7%) characterized only to genus level. These total sequences in RDP showed homology with ~60% of uncultured phylotypes. Therefore, the sequences analyzed with HOMD were taken into consideration for species level identification. The venn diagrams (Figure 5) are embedded to corresponding section of pie chart except for the unclassified sequences and the inset values in two subsets (non-tumor and tumor) correlates to observed selleck chemicals llc bacterial species unique to that particular library. The number of species shared or common to both the groups is seen in overlapping section of subsets. Figure 5 Relative distribution of total bacteria (cultivable species Blebbistatin concentration and uncultured phylotypes) in tissues from non-tumor and tumor sites of OSCC subjects characterized by HOMD. Core of pie chart shows percentage distribution of total 914 filtered sequences in terms of their % homology to curated 16S rRNA sequences in HOMD. Outer concentric of pie chart depicts the oral bacterial taxa in combined library; sequences with >98% identity: named cultured species (78.6%), unnamed cultured species (5.9%) and yet-uncultured phylotypes (3.8%); and sequences with <98% identity (11.7%) were this website considered as unclassified sequences characterized only to genus level.

Venn diagrams correlates with the corresponding section of pie chart as indicated by line except

for the unclassified sequences. Inset values in two subsets (non-tumor and tumor) represents observed bacterial species unique to that particular library. Values in overlapping section of subsets reflect oral taxa common to both sites. In total, 80 bacterial species/phylotypes were detected, 57 in non-tumor and 59 in tumor library. The unnamed cultivable biota, Actinomyces sp. oral taxon Monoiodotyrosine 181, phylotype Leptotrichia sp. oral taxon 215, and certain named bacterial species, Prevotella histicola, Prevotella melaninogenica, Prevotella pallens, Fusobacterium nucleatum ss. nucleatum, Escherichia coli and Neisseria flavescens were detected at non-tumor site while Atopobium parvulum and Fusobacterium nucleatum ss. vincentii at tumor site (Figure 6a). The microbiota associated with phylum Firmicutes showed interesting switch in profile (Figure 6b). Species, Granulicatella adiacens, Mogibacterium diversum, Parvimonas micra, Streptococcus anginosus, Streptococcus cristatus, Streptococcus mitis and Veillonella dispar were prevalent at non-tumor site of the OSCC patients. The unnamed cultivable taxon, Streptococcus sp. oral taxon 058, and named cultivable bacterial species, Gemella haemolysans, Gemella morbillorum, Gemella sanguinis, Johnsonella ignava, Peptostreptococcus stomatis, Streptococcus gordonii, Streptococcus parasanguinis I, Streptococcus salivarius were highly associated to tumor site.