In terms of robustness study for a HPLC assay, analytical column is one of the most typical changing variables. To give some freedom in the method, different columns from various manufacturers were tested, by applying the same chromatographic conditions; baseline separations of the steroids tested were independent of column brand, demonstrating good robustness of the method. The above results were considered to be satisfactory for subsequent quantitative analysis of commercial samples. buy Volasertib After satisfactory development of method it was subject to method validation as per ICH guideline.16 and 17 The method was validated to demonstrate that it

is suitable for its intended purpose by the standard procedure to evaluate adequate validation characteristics (system suitability, accuracy, precision, linearity, robustness, ruggedness, solution stability, LOD and LOQ and stability indicating capability and parameters like theoretical plates, tailing factor and resolution for the standard solution were also calculated and indicated in Table 7). The proposed HPLC-PDA and ELSD method was successfully applied to simultaneous determination of steroids from commercial source. AZD2281 chemical structure The qualitative analyses were performed by means of the external standard methods and the

LCMS, HPLC-PDA and ELSD chromatograms of all samples are presented in Figs. 1 and 2. The present paper describes the development of a new LCMS method for the determination of three steroidal hormone drugs i.e. Dexamethasone; Testosterone and Estrone (E1) under Thiamine-diphosphate kinase three sets of analytical conditions. We could be able to get more and authentic information as chromatograms were recorded using ELSD detector in addition

to using Photon diode array detector followed by recording high resolution mass spectra. LCMS method was found to be more specific than those HPLC methods reported in pharmacopoeias. The method was found to be selective, sensitive, precise and accurate for the determination of steroids. The method was shown to be very useful for routine applications in the field of pharmaceutical analysis, especially steroidal hormone drugs. All authors have none to declare. The authors are wish to express their gratitude to the management of Evolva Biotech Pvt. Ltd, Chennai, India for providing necessary facilities to carry out the research. “
“Polycyclic aromatic hydrocarbons (PAHs) are fused ring aromatic compounds and considered as major pollutants in the environment.1 They are produced during incomplete oxidation of different organic molecules. In the environment they have five distinct fates: volatilization, leaching, degradation, bioaccumulation and sequestration. However due to their high chemical stability2 and hydrophobicity3 they are difficult to be removed from the environment by the common physicochemical methods4; they remain suspended in aquatic environment and ultimately they enter the food chain5 causing harmful effects on us.