We assumed participants Cisplatin manufacturer moved during July of the indicated moving year. As in our previous studies, we calculated each months average daily insolation and temperature exposure at each participants Inhibitors,Modulators,Libraries residential location to estimate each participants average exposure for the year previous to baseline. We then categorized insolation and temperature exposure into quartiles. In order to capture extreme exposures, we Inhibitors,Modulators,Libraries also categorized insolation and temperature exposure using cutpoints at the 5th and 95th percentiles. Outcomes Blood pressure was measured during the REGARDS in home visit by a trained technician using a standard protocol and regularly tested aneroid sphygmomanometer and was calculated as an average of two measurements taken after the participant was seated for five minutes.

Hypertension was defined as either self reported Inhibitors,Modulators,Libraries use of antihypertensive medications or a systolic blood pressure 140 mm Hg or a diastolic blood pressure 90 mm Hg. Blood was collected during the in home visit, and shipped to the central laboratory at the Inhibitors,Modulators,Libraries University of Vermont using standard protocols. Standard assays were used to determine lipid levels and high sensitivity C reactive protein assays were used to determine the level of CRP, which was log transformed due to a skewed distribution. CRP levels were categorized into low medium risk and high risk. Dyslipidemia was defined as self reported use of lipid lowering medication or total cholesterol 240 mg dL or low density lipoprotein 160 mg dL or high density lipoprotein 40 mg dL. Kidney function was determined by the estimated glomerular filtration rate computed using the CKD EPI equation.

Statistical methods Because some confounders had missing data Inhibitors,Modulators,Libraries for large numbers of participants, we attempted to minimize selection bias by creating a separate missing category for any variable that had 1,000 participants missing data. Participants were excluded due to data anomalies, stroke or coronary heart disease at baseline, missing residential history, and missing confounder data. Of the 30,239 participants never enrolled at baseline, 17,773 participants were available for analyses. To perform a split sample replication analysis we randomly assigned the eligible participants into one of two samples of equal size. In the first exploratory sample, we ran multivariable logistic or linear regression models adjusting for temperature, age, race, region, gender, education, income, quartiles of vitamin D intake, exercise, alcohol use, smoking status and body mass index. We also adjusted for statin use in models with cholesterol, HDL, or LDL as the outcome, and adjusted for antihypertensive medication use in the models with SBP as the outcome.