RESULTS: Information was obtained for 113 of 115 centres for adolescents and 72/72 centres for children. Both age groups: most centres using passive consent achieved high response rates (>80% adolescents and >70% children). English language centres using active consent showed a larger decrease in response rate. Ado-lescents: seven centres changed from passive consent in Phase I to active consent in Phase III (median decrease of 13%), with five centres showing lower response rates (as low as 34%). Children: no centre changed consent method
between phases. Centres using active consent had lower median response rates (lowest response rate 45%).
CONCLUSION: The requirement for active consent for population school-based questionnaire studies can impact negatively on response rates, particularly English language centres, thus adversely affecting the validity GSI-IX of the data. Ethics committees need to VX-661 in vivo consider this issue carefully.”
“Aim: The aim of this study was to explore the effect of Src-homology-2-domain-containing protein, tyrosine phosphatase 2 (SHP-2) on the proliferation of cervical cancer cells. Material and Methods: A total of 45 patients with cervical cancer (stage IIII), 32 with cervical intraepithelial neoplasia and 20 healthy subjects
were consecutively recruited. The levels of SHP-2 and interferon (IFN)-beta expression in cervical tissues were characterized by immunohistochemistry and statistically analyzed by logistic regression. Following knockdown of SHP-2
expression by a siRNA or pre-treatment with a specific peptide, the effect of SHP-2 expression in THP-1 cells on the growth and survival of SiHa cells and on IFN-beta production was determined by co-culture assays, 3-(4,5)-dimethylthiazol (-z-y 1)-3,5-diphenyltetrazolium bromide, and enzyme immunosorbent assay. Results: selleck products The levels of SHP-2 expression in cervical cancer tissues were significantly higher than that in cervical intraepithelial neoplasia and uterine myoma tissues (P < 0.05, respectively), and negatively correlated with the levels of IFN-beta expression in these tissues (R = -0.582, P < 0.05). Knockdown of SHP-2 expression with SHP-2 siRNA or treatment with the SHP-2-specific blocking peptide in THP-1 cells significantly increased the production of IFN-beta (P < 0.05, respectively) and inhibited the proliferation of SiHa cells in a co-culture system of THP-1 and SiHa cells (P < 0.05, respectively). Conclusions: SHP-2 phosphatase promotes cervical cancer cell proliferation through inhibiting IFN-beta production.”
“In India, the Revised National Tuberculosis Control Programme and a large-scale human immunodeficiency virus (HIV) prevention project partnered to deliver enhanced TB screening services for HIV high-risk groups.